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Journal / Developmental Biology / 无支架三维胰岛素表达 Pancreatoids…
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Developmental Biology

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JoVE Journal Developmental Biology
无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞
 
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无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞

Article DOI: 10.3791/57599-v • 09:33 min • June 2nd, 2018
June 2nd, 2018 •
1, 2,3,4, 2,3,4, 1,2,3,4,5
1Program in Developmental Biology, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, 3Molecular and Cellular Biology Department, Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, 5McNair Medical Institute, Baylor College of Medicine

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Summary
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在这里, 我们提出了一种生成胰岛素表达3D 小鼠 pancreatoids 从游离漂浮 e10.5 分离的胰祖细胞和相关间质的协议。

Tags

发育生物学 问题 136 胰腺 Organoids β细胞 发育 间质 分化 糖尿病 3D 文化 Pancreatoids
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