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1Department of Internal Medicine-Gastroenterology,University of Michigan, 2Department of Molecular and Integrative Physiology,University of Michigan, 3Department of Molecular, Cellular and Developmental Biology,University of Michigan, 4Department of Gastrointestinal Surgery,The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology,University of Arizona College of Medicine
Kapitel
- 00:04Titel
- 00:33Dissection of the Intestinal Segment
- 03:01Removal of the Epithelial Mucosa
- 04:57Collection of Enteric Glial Cells from the Lamina Propria and Submucosa
- 06:23Results: Enteric Glial Cells 24 h After Plating
- 07:12Conclusion
Burada, bağırsak submukoza divalent katyonlar ve enzimatik olmayan hücre kurtarma çözümü kuluçka şelasyon yapın için sıralı EDTA incubations kullanarak gliyal enterik hücre izolasyon açıklayın. Sonuç hücre süspansiyon poli-D-lizin ve laminin kaplama submucosal Gliyal hücreler fonksiyonel analiz için zenginleştirilmiş bir kültür sonuçlanır.
