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Journal / Cancer Research / Verrijking van de Phosphopeptide in combinatie…
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Cancer Research

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JoVE Journal Cancer Research
Verrijking van de Phosphopeptide in combinatie met kwantitatieve Label-vrije Spectrometrie van de massa te onderzoeken van de Phosphoproteome in prostaatkanker
 
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Verrijking van de Phosphopeptide in combinatie met kwantitatieve Label-vrije Spectrometrie van de massa te onderzoeken van de Phosphoproteome in prostaatkanker

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

Kapitel

Summary
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Dit protocol beschrijft een procedure om uitpakken en verrijken van phosphopeptides van prostaatkanker cellijnen of weefsels voor een analyse van de phosphoproteome via massa spectrometrie gebaseerde proteomics.

Tags

Kankeronderzoek kwestie 138 prostaatkanker massaspectrometrie proteomics Fosfoproteomics fosforylatie kinases cel signalering
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