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JoVE Journal Medicine
Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
 

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Article DOI: 10.3791/59052-v • 11:26 min • February 13th, 2019
February 13th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 1,3, 1
1Division of Cardiology, Department of Medicine, University of California San Diego, 2Department of Pharmacology, University of California San Diego, 3Cardiology Section, Department of Medicine, Veterans Administration Healthcare, San Diego

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Summary
Automatic Translation

The goal of this protocol is to illustrate how to use mouse neonatal cardiomyocytes as a model system to examine how various factors can alter oxygen consumption in the heart.

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Oxygen Consumption Rate Primary Cultured Mouse Neonatal Cardiomyocytes Extracellular Flux Analyzer Mitochondrial Function Adherent State 96-well Format Multiple Conditions Replicates Cardiology Research Metabolic Parameters Greygrices Peracid Epoxidation Variability Of Cardiomyocytes Digestion Of Hearts Fragile Cardiomyocytes Gentle Handling HBSS Solution Trypsin Predigestion Solution Sterile Conical Tube
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