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Genetics

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JoVE Journal Genetics
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
 

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Summary
Automatic Translation

This protocol outlines experimental procedures to characterize genome-wide changes in the levels of histone post-translational modifications (PTM) occurring in connection with the overexpression of proteins associated with ALS and Parkinson's disease in Saccharomyces cerevisiae models. After SDS-PAGE separation, individual histone PTM levels are detected with modification-specific antibodies via Western blotting.

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Characterizing Histone Post-translational Modification Alterations Yeast Neurodegenerative Proteinopathy Models Protocol Epigenetic Features Neurodegenerative Disease Yeast Model System Cellular Models Animal Models Cost-efficient Map Out Epigenetic Changes Markers For Diagnosis New Targets For Therapy Investigation Role Of Epigenetic Marks Human Fibroblasts Induced Pluripotent Stem Cells Assessment Western Blot Apparatus Restreaking Yeast Frozen Glycerol Stocks Histidine 2%glucose Agar Selective Medium Plates Two To Three Day Incubation 30 Degrees Celsius Inoculate Restreaked Yeast Over Expression Model Control Five Milliliters Of Histidine Selective Liquid Medium
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