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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16
 

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Article DOI: 10.3791/59920-v • 06:03 min • July 15th, 2019
July 15th, 2019 •
1, 2, 3,4, 3,5
1Department of Molecular Biosciences, University of Texas at Austin, 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 3Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 4Department of Microbiology and Immunology, Dalhousie University, 5Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University

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Summary
Automatic Translation

The goal of the protocol is to illustrate the different assays relating to viral entry that can be used to identify candidate viral entry inhibitors.

Tags

Viral Entry Assays Molecular Docking Analysis Antiviral Candidates Coxsackievirus A16 Small Molecules Mechanism-driven Approach Time Of Addition Assay Antiviral Activity Viral Proteins Drug Evaluation On Host Cells RD Cells Plating Density Test Compound Non-toxic Concentrations Basal Medium Virus Infection Plaque-forming Units Rocking Methylcellulose Cell Culture Incubator Formaldehyde Fixation
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