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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
 

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Article DOI: 10.3791/60585-v • 08:02 min • January 22nd, 2020
January 22nd, 2020 •
1,2, 1, 1, 1,3,4, 1,3
1Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 4Jinan Infectious Disease Hospital, Shandong University

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Summary
Automatic Translation

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

Tags

In Vivo Augmentation Gut-homing Regulatory T Cell Induction Therapeutic Strategy Human Applications Peripheral Lymphoid Tissues Pro-inflammatory Environments Active Vitamin D Vitamin A Engineered Dendritic Cells CM-10-D Cell Culture Medium 293T Cells HBS Solution Envelope Plasmid Packaging Plasmid LENTI-CYP-ALDH Plasmid Calcium Chloride
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