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JoVE Journal Medicine
Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber
 

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

Article DOI: 10.3791/61234-v • 09:07 min • June 11th, 2020
June 11th, 2020 •
1,2, 1,2, 1,2
1Department of Experimental Medical Science, Lund University, 2Lund University Diabetes Centre

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Summary
Automatic Translation

The goal of this protocol is to continuously monitor the dynamics of the human pancreatic islet engraftment process and the contributing host versus donor cells. This is accomplished by transplanting human islets into the anterior chamber of the eye (ACE) of an NOD.(Cg)-Gt(ROSA)26Sortm4-Rag2-/-mouse recipient followed by repeated 2-photon imaging.

Tags

Longitudinal In Vivo Imaging Quantification Human Pancreatic Islet Grafting Contributing Host Cells Anterior Eye Chamber ACE Technique Sub-cellular Resolution Beta Cell Replacement Therapy Islet Grafting Efficiency Islet Survival Islet Transplantations Diabetic Complications Kidney Glomeruli Liver Pieces Mouse Positioning Head Holder Nose Mask Buprenorphine Injection Eye Transplantation
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