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低温样品加载到魔法角度旋转核磁共振光谱仪中，可保持细胞生存能力

JoVE Journal
Biologie

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JoVE Journal Biologie

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Please note that all translations are automatically generated. Click here for the English version.

低温样品加载到魔法角度旋转核磁共振光谱仪中，可保持细胞生存能力

DOI:

10.3791/61733-v

•

06:42 min

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September 01, 2020

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Rupam Ghosh*¹, Jaka Kragelj*¹, Yiling Xiao*¹, Kendra K. Frederick²

¹Department of Biophysics,University of Texas Southwestern Medical Center, ²Center for Alzheimer’s and Neurodegenerative Disease and Center for Systems Biology,University of Texas Southwestern Medical Center

Kapitel

00:04Introduction
00:49Cryopreservation of Mammalian Cells in an NMR Rotor
01:25Cryogenic Transfer of a Frozen Sample into the NMR Spectrometer
03:48Cryogenic Removal of the Sample from the NMR Spectrometer
05:02Results: Cryogenic Insertion of a Pre-frozen Sample of Mammalian Cells Results in Cellular Intactness Similar to Never-frozen Mammalian Cell Samples
05:47Conclusion

Summary

Automatische Übersetzung

这里介绍了冷冻样品低温传输到动态核极化（DNP）神奇角度旋转（MAS）核磁共振（NMR）探测器的协议。该协议包括实验前转子存储方向和实验前后可行性测量方向。

Tags

Cryogenic Sample Loading Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer Cellular Viability DNP Assisted MAS NMR Protein Characterization Sample Handling Cryogenic Transfer Rotor Liquid Nitrogen Microcentrifuge Tube NMR Sample Catcher Spectrometer Facility

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