Journal
/
Biologie
/
加载编码RNA核酸适体和核糖开关的DNA质粒的多功能丝基微胶囊的制备
JoVE Journal
Biologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biologie
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Please note that all translations are automatically generated. Click here for the English version.

加载编码RNA核酸适体和核糖开关的DNA质粒的多功能丝基微胶囊的制备

DOI:
10.3791/62854-v

•

10:07 min

•

October 08, 2021

•

, , ,
1UES Inc., 2711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory,Wright-Patterson AFB, 3Materials and Manufacturing Directorate,Wright-Patterson AFB

Kapitel

  • 00:04Introduction
  • 00:56Perform Layer‐by‐Layer Deposition of a Prime Layer, DNA Plasmids, and Silk Layers
  • 04:44Dissolution of SiO2Cores and Imaging of Silk Fibroin Microcapsules
  • 05:42Estimation of the Permeability of Hollow Microcapsules Using Molecular Weight Cut‐Off (MWCO) Method
  • 06:42In Vitro Activation of Synthetic Theophylline Riboswitch in Silk Microcapsules
  • 07:28Results: Microscopy Studies, Membrane Permeability, and Gene Expression Kinetics of Silk Fibroin Microcapsules
  • 09:25Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

该协议描述了稳健且生物相容的富含DNA的微胶囊的形成，作为能够跟踪多种配体的多重体 外 生物传感器。

Tags

Multifunctional Silk-based MicrocapsulesDNA PlasmidsRNA AptamersRiboswitchesBiocompatible MicrocapsulesDNA ImmobilizationBiosensorsBiological ProcessesSignaling MoleculesGene ActivationPolyethyleneimineSilica MicroparticlesDNA PlasmidsTheophylline RiboswitchBroccoli AptamerSilk Fibroin

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

自制的网站定向诱变整个质粒

可视化的单分子荧光显微镜下的DNA复制

改造使用含RNA寡核苷酸基因组DNA的RNA / DNA杂交一代

iCLIP - 全转录组，蛋白质与RNA相互作用的映射与个别核苷酸分辨率

在体外转录和Gaussia荧光素酶基因的上限由HeLa细胞转染

DNA交联聚丙烯酰胺水凝胶的制备

单工程的RNA转录物的活细胞实时成像采用比例式双分子信标

样机小型化流式细胞仪及配套的混合微流控技术降低重力环境硬件演示

逆转录病毒整合位点的扩增，新一代测序和基因组DNA制图

表面拴DNA大分子的可视化与荧光蛋白的DNA结合肽

Read Article