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Journal / Biology / Primary Human Nasal Epithelial Cells: Biobanking…
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JoVE Journal Biology
Primary Human Nasal Epithelial Cells
 

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

Article DOI: 10.3791/63409-v • 08:35 min • April 22nd, 2022
April 22nd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2,3
1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées, Assistance Publique Hôpitaux de Paris

Kapitel

Summary
Automatic Translation

Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.

Tags

Primary Nasal Epithelial Cells Biobanking Precision Medicine Culture Conditions Patient-derived Cultures Personalized Medicine Studies CFTR Activity Cystic Fibrosis Therapy Nasal Epithelial Or HNE Cells Amplification Medium 0.25% Trypsin Cell Detachment
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