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Journal / Biology / Lentiviral Mediated Delivery of shRNAs to hESCs…
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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
 

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Summary
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Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.

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Lentiviral Mediated Delivery ShRNAs HESCs NPCs Cationic Polymer Polyethylenimine (PEI) Transfection HEK293T Cells Low Protein Binding Filter Lentiviral Vector Filtration Sucrose Cushion Ultracentrifugation DMEM Medium FBS Penicillin Streptomycin 37 Degrees Celsius Humidified Incubator Carbon Dioxide Oxygen 90% Confluency Culturing Growth Medium Tissue Culture Plate Plasmid DNA Stocks Serum-free DMEM Media Centrifuge Tubes Entry And Packaging Plasmids Lipofectamine-based Transfection
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