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Establishing 3D Endometrial Organoids from the Mouse Uterus
 

Establishing 3D Endometrial Organoids from the Mouse Uterus

Article DOI: 10.3791/64448-v • 06:24 min • January 6th, 2023
January 6th, 2023 •
1,2, 1,2, 1,2, 1,2, 3, 1,2
1Department of Pathology & Immunology, Baylor College of Medicine, 2Center for Drug Discovery, Baylor College of Medicine, 3Departments of Molecular Virology and Microbiology and Medicine, Baylor College of Medicine

Kapitel

Summary
Automatic Translation

This protocol describes methodologies to establish mouse endometrial epithelial organoids for gene expression and histological analyses.

Tags

3D Endometrial Organoids Mouse Uterus Uterine Epithelium Culture Isolation Enzymatic Dissociation Mechanical Dissociation Gel Matrix Dissecting Euthanized Mouse Midline Incision Abdominal Fat Pads Cervical Junction Mesenteric Fat Small Scissors Uterine Fragments 24 Well Plate Trypsin Incubation
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