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Journal
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Biologie
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通过冷冻STEM断层扫描原
位
可视化细胞器
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Visualization of Organelles
In Situ
by Cryo-STEM Tomography
Please note that all translations are automatically generated.
Click here for the English version.
通过冷冻STEM断层扫描原
位
可视化细胞器
DOI:
10.3791/65052-v
•
08:37 min
•
June 23, 2023
•
Peter Kirchweger
,
Debakshi Mullick
,
Sharon Grayer Wolf
,
Michael Elbaum
1
Department of Chemical and Biological Physics
,
Weizmann Institute of Sciences
,
2
Department of Chemical Research Support
,
Weizmann Institute of Sciences
Kapitel
00:05
Introduction
00:48
STEM Setup
04:37
Placing the Specimen and Recording an Image
06:58
Recording an Image
07:23
Results: Visualization of the Organelles
08:03
Conclusion
Summary
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Automatische Übersetzung
冷冻 STEM 断层扫描提供了一种可视化完整细胞细胞器的方法,无需包埋、切片或其他侵入性制剂。获得的3D分辨率目前在几纳米的范围内,视场为几微米,可访问厚度约为1微米。
Tags
Cryo-STEM Tomography
TEM Tomography
FIB SEM
Soft X-ray Tomography
STEM Optics
Column Alignment
Eucentric Focus
STEM Mode
Condenser Aperture
Beam Shift
Rotation Center
Pivot Points
Condenser Stigmators
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