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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
 

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Article DOI: 10.3791/737-v • 08:51 min • May 27th, 2008
May 27th, 2008 •
1, 2, 2
1Department of Evolutionary Biology of Invertebrates, University of Tubingen, 2European Synchrotron Radiation Facility

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Summary
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We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.

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Non-invasive 3D Visualization Sub-micron Resolution Synchrotron-X-ray-tomography Micro-arthropods Internal Organization Small Size Hard Cuticle Classical Histology Non-destructive Method Synchrotron X-ray Tomography European Synchrotron Radiation Facility (ESRF) Grenoble (France) 3D Tomographic Datasets Pixel-resolution Volume Rendering Software Quantitative Morphology Landmarks Animated Movies Hidden Body Parts Complete Organ Systems Tissues
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