Image Analysis in Gen5 Software to Analyze Drug Response in Patient-Derived Organoids Using Multiplexed Live-Cell Imaging - JoVE

JoVE Journal > Cancer Research

Please note that all translations are automatically generated. Click here for the English version.

Investigación sobre el cáncer

Анализ изображений в программном обеспечении Gen5 для анализа реакции на лекарственные препараты в органоидах, полученных от пациентов, с использованием мультиплексной визуализации живых клеток

Published: January 05, 2024

doi:

Kaitriana E. Colling*^1,2, Emily L. Symons*¹, Lorenzo Buroni*³, Hiruni K. Sumanasiri, Jessica Andrew-Udoh, Emily Witt⁴, Haley A. Losh, Abigail M. Morrison, Kimberly K. Leslie, Christopher J. Dunnill, Johann S. de Bono, Kristina W. Thiel⁷

¹Department of Obstetrics and Gynecology, Carver College of Medicine,University of Iowa, ²Cancer Biology Graduate Program, Carver College of Medicine,University of Iowa, ³The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust, ⁴Department of Radiation Oncology, Carver College of Medicine,University of Iowa, ⁵Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology,University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center, ⁶Agilent Technologies, ⁷Holden Comprehensive Cancer Center,University of Iowa

Tags

Keywords: Image Analysis Gen5 Software Drug Response Patient-derived Organoids Multiplexed Live-cell Imaging Cytation 5 Z Projection Cellular Analysis Object Selection Subpopulation Analysis Object Metrics Cellular Debris Single Cells Circularity

Play Video

DOI

Citar este artículo

Image Analysis in Gen5 Software to Analyze Drug Response in Patient-Derived Organoids Using Multiplexed Live-Cell Imaging. J. Vis. Exp. (Pending Publication), e201046, doi:10.3791/201046 (2024).

View Video