Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells

Published: April 30, 2023

Abstract

Source: Hossian, A. et. al. Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis. J. Vis. Exp. (2019).

This video describes the technique to study the protein expression in miRNA transfected lung cancer cells by using antibody microarray to measure specific gene expression. In the featured protocol, we perform antibody microarray for the identification of the activity of a combinatorial miRNA treatment in halting cell cycle and angiogenesis.

Protocol

1. miR-143 and miR-506 transfection CAUTION: Use latex gloves, protective eyeglasses, and a laboratory coat while performing the described experiments. When required, use the biosafety cabinet with the cabinet fan on, without blocking the airways or disturbing the laminar airflow. Always set the protecting glass window to the appropriate height, as described by the manufacturer. Seed NSCLC A549 cells in a T25 cm2 flask/6/96 well plate in DMEM/F12K m…

Divulgaciones

The authors have nothing to disclose.

Materials

A549 Non Small Cell Lung Cancer Cells   ATCC ATCC CCL-185
Penicillin-streptomycin 10/10  Antlanta Biologicals B21210
Fetal Bovine Serum (FBS)   Fisher Scientific 10438026
Antibody Array Assay Kit, 2 Reactions  Full Moon Bio  KAS02
Cell Cycle Antibody Array, 2 Slides   Full Moon Bio ACC058
ScanArray Express   PerkinElmer Step 2.33: Microarray analysis software
HyClone Phosphate Buffered Saline (PBS)   Fisher Scientific SH30256FS
Thermo Scientific Pierce BCA Protein Assay   Thermo Scientific PI23226

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Citar este artículo
Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells. J. Vis. Exp. (Pending Publication), e20239, doi: (2023).

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