Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue

Abstract

Source: Heavey, S. et al. Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking. J. Vis. Exp. (2019)

This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Prostate Slicing Apparatus Purchase the prostate slicing apparatus (Table of Materials). Alternatively, print a blade handle using a 3D printer. 2. Tumor Targeting <l…

Representative Results

Figure 1: Prostate slicing apparatus.  (A) The walls are inserted perpendicular to the base, and gold pins are inserted into the base surrounding the prostate (prostate not pictured). (B) The replaceable parallel blades are inserted into the blade handle. (C) The blades pass between the gold pins in order to slice a 5 mm section of the prost…

Materials

6 mm biopsy punch	Fisher Scientific	13404607	Disposable biopsy punches for removing 6 mm tissue samples
Black Ink	Leica Biosystems	3801753	Tissue marking & margin dye
Blue Ink	Leica Biosystems	3801751	Tissue marking & margin dye
Chainmail hand glove	Arco	1456803	Chainmail gloves to protect hand during slicing
Cork board	Fisher Scientific	12396447	Cork board for pinning prostate to following sampling procedure
Needles	SLS (Scientific Laboratory supplies)	SYR6112	Sterile needles to use to pin tissue to cork board following sampling
Prostate slicing aparatus	Insitute of Cancer Research, London	NA – must be obtained under MTA	A kit containing the slicer handle, blades, spacer, base, walls and pins