Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
Published: April 30, 2023
Abstract
Source: Sugimoto, K. et al. Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer. J. Vis. Exp. (2020)
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Washing the Slides Prepare 10 µm of unstained formalin-fixed paraffin-embedded (FFPE) sections. Place slides in a glass slide holder: use about 3-5 largest cross-sectional slides unless the tissue is minimal, and more slides are …
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The authors have nothing to disclose.
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Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration. J. Vis. Exp. (Pending Publication), e20408, doi: (2023).