Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model

Published: April 30, 2023

Abstract

Source: Bonetto, A. et al. The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia. J. Vis. Exp. (2016)

In this video, we describe the procedure to excise hindlimb skeletal muscles from a mouse model. The excised muscles can be stored at lower temperatures until further downstream analysis. 

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tissue and Organ Excision NOTE: For use of tissues in biochemical or molecular biology assays, plan to weigh each organ and tissue and place a fragment immediately into pre-labeled cryotubes. Snap freeze in liquid nitrogen and store at -80 °C. In order to avoid contamina…

Divulgaciones

The authors have nothing to disclose.

Materials

CD2F1 mice Harlan 60
Anesthesia apparatus EZ-Anesthesia EZ-7000
OCT Tissue-Tek 4583
Cryostat Leica CM1850
Cork disks Electron Microscopy Sciences 63305
Microtainer BD  365873
Superfrost plus glass slides VWR 48311-703
Streptomycin-Penicillin Cellgro 30-002-CI

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Hindlimb Skeletal Muscle Extraction: A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model. J. Vis. Exp. (Pending Publication), e20422, doi: (2023).

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