In this video, we demonstrate a procedure to isolate exosomes from target biofluids using magnetic microbeads. The procedure enables the rapid capture of exosomes, which can be used for downstream analysis.
Protocol
1. Magnetic Bead-based Exosome Extraction Pipette a well-mixed solution of 5 µL of Streptavidin-coated magnetic microparticles into 495 µL of phosphate-buffered saline (PBS) buffer in a microcentrifuge tube to resuspend the beads. Wash and resuspend the beads with 500 µL of PBS three times using a magnetic rack. The rack is an array of magnets on the side of a housing unit that can hold the sample microcentrifuge tubes. For each wash, first, let the tubes sit on the…
Magnetic Bead-based Exosome Extraction: A Technique to Isolate Specific Exosomes from Biofluids. J. Vis. Exp. (Pending Publication), e20496, doi: (2023).