Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain

Published: April 30, 2023

Abstract

Source: Geisler, J. A. et al. Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging. J. Vis. Exp. (2020)

This video demonstrates implantation of tumor cells in the mouse brain by performing intracranial injection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Injecting Cancer Cells into the Brain Place an anesthetized mouse with an exposed calvarial window on the stereotatic frame. Attach an automatic injector unit to the stereotactic apparatus (Figure 1). Pull up >2 µL of cells thoroughly resuspended in 1x DPBS in a clean Hamilt…

Representative Results

Figure 1: Stereotactic and anesthesia systems. Stereotactic stand with automated syringe pump and mouse elevating box. The box is an inverted pipette tip box containing hand warmers used to elevate the mouse to the appropriate height and maintain body temperature. Inset shows the orientation of the Hamilton syringe in the automated injection apparatus.

Divulgaciones

The authors have nothing to disclose.

Materials

Bone Wax  Surgical Specialities  903 Sterile and malleable beeswax and isopropyl palmitate
Cotton tip applicators  Puritan  25-806 10WC  Sterile long stemmed cotton tip applicators
Suture  Covidien  SP5686G  45 cm swedged 5-0 monofilament polypropylene suture
Skin Glue  Vetbond  1469SB  Skin safe wounds adhesive
Stereotaxic Unit
Mouse Gas Anesthesia Head Holder  Kopf  Model 923-B  Mouth bar with teeth hole and nosecone
Non-Rupture Ear Bars  Kopf  Model 922  Ear bars suitable for mouse applications
Stereotaxic Instrument  Kopf  Model 940  Base plate, frame and linear scale assembly with digital readout monitor
Injector
Injector Needle and syringe Hamilton 80366 26 gauge needle, 51 mm needle length and 10 μL volume syringe
Legato 130A automated Syringe Pump  KD Scientific  P/N: 788130  Programmable touch screen base with automated injector
Anesthesia Machine 
SomnoSuite Low-Flow Digital Vaporizer Kent Scientific  SS-01  Digital anesthesia machine

Tags

Play Video

Citar este artículo
Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain. J. Vis. Exp. (Pending Publication), e20610, doi: (2023).

View Video