Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection

Published: April 30, 2023

Abstract

Source: Zhang, C. et al. Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis. J. Vis. Exp. (2017)

This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Prepare Mice for Tumor Cell Injection Anesthetize a mouse by intraperitoneal (i.p.) injection of ketamine/xylazine cocktail (ketamine 100 mg/kg, xylazine 10 mg/kg). Confirm complete anesthesia by pinching the animal's feet and observe lack of response. Place the mouse on a glass p…

Divulgaciones

The authors have nothing to disclose.

Materials

Ketamine hydrochloride/xylazine hydrochloride solution Sigma-Aldrich K113
Routine Stereomicroscope Leica M50 Leica M50 The microscope is modular and highly configurable to fit particular space requirements
Surgical disposable scapel Integra Miltex 4-410 To make skin incisions
Tissue Forceps – 1 x 2 Teeth Fine Science Tools 11021-12 To bluntly dissect muscles
Dumont #5 – Mirror Finish Forceps Fine Science Tools 11252-23 To separate and prepare the carotid artery for injection
Spring Scissors Fine Science Tools 15025-10 To cut sutures
EZ Clip Kit Stoelting 59020 For wound closure
BD Insulin Syringe Becton Dickinson 328438 For cell injection
IVIS Spectrum In Vivo Imaging System PerkinElmer 124262

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Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection. J. Vis. Exp. (Pending Publication), e20688, doi: (2023).

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