Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion

Published: April 30, 2023

Abstract

Source: Serra, M. B. et al. Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice. J. Vis. Exp. (2021)

This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Laparotomy Anesthetize animals with xylazine (10 mg/kg) and ketamine solution (80 mg/kg), subcutaneously (0.1 mL/10g of body weight) using a 1 mL syringe and 13 x 0.45 mm needle 26 G ½. Check for sufficient anesthesia depth by pinching the toe. Control body temperature using heated pads. Ensure that all surgical ma…

Representative Results

Figure 1: Schematics of severe acute pancreatitis induction by 2.5% sodium taurocholate in C57BL/6 mice. (A) gallbladder; (B) common bile duct; (C) pancreatic duct; (D) portal vein; (E) microvessel clip; (F) puncture site (needle attached to a polyethylene tube an…

Divulgaciones

The authors have nothing to disclose.

Materials

0.4 mm needle INTRAG MEDICAL TECH 90183210 30G
0.54 mm polyethylene tube Tygon 730010
Styrofoam block
Masking tape for mounting the mouse Missner 1236
Infusion pump scheduled to 10µL/min. Havard aparatus-Peristaltic Pump Series MA1 55-7766  Model 66 Small Peristaltic
Scissors and forceps
Antiseptic providine iodine Pfizer 12086OR Antisepsis
70% ethanol SIGMA 459836 Mix 700 mL 100% ethanol with 300 mL dH2O
Razor blade Lord bdk9a1ghk6 For trichotomy
Sodium taurocholate Sigma-Aldrich 86339- 1G CAS NUMBER- 345909-26-4
Microvessel clip Medicon Surgical 56.87.35 Approximator, opening 4.0 mm, closing pressure 30-40 g
6-0 prolene Bioline 5162 Suture line
Ketamin NP (cloridrato de dextrocetamina) 50mg/mL Cristália
Xilazine 2% Syntec
Sterile saline solution (0.9% (wt/vol) saline) Farmace 105851
Methyl Blue Sigma-Aldrich Chemicals M5528
Desmarres retractor 13 mm width ROBOZ RS-6672

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Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion. J. Vis. Exp. (Pending Publication), e20750, doi: (2023).

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