This video demonstrates the role of vitronectin, a human serum glycoprotein, on bacterial resistance. Antibodies in the serum interact with bacterial surface proteins, activating complement proteins and forming the membrane attack complex, causing bacterial cell lysis. Vitronectin binding on bacterial membrane protein prevents the membrane attack complex formation, resulting in bacterial survival.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum Purchase normal human serum (NHS) from a commercial source. Prepare vitronectin or Vn-depleted serum (VDS). Replenish the VDS with 180 nM Vn which is equivalent to Vn present in NHS. Prepare heat-inactivated serum (HIS) by heating NHS at 56 °C for 30 mi…
Determining the Role of Vitronectin in Bacterial Resistance to Bactericidal Activity of Human Serum. J. Vis. Exp. (Pending Publication), e21697, doi: (2023).