Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice

Abstract

Source: Zychlinsky Scharff, A., et al. Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice. J. Vis. Exp. (2017).

This video demonstrates the establishment of uropathogenic Escherichia coli infection in a female mouse bladder through transurethral catheter instillation. The injected bacteria successfully establish a urinary tract infection within the bladder, providing a valuable platform for studying healthcare-associated diseases related to the urinary tract.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Catheters Prepare one pediatric intravenous-access cannula for each group of mice to be infected. Using the inbuilt spring mechanism, divest each cannula of its needle, as instructed by the manufacturer. Discard the needles, preserving only the plastic intravenous cannula. Sterilize catheters in…

Representative Results

Figure 1: The Quality of Instillation does not Correlate with Bacterial Burden at 24 hr Post-infection. Six to 8 week-old female and male C57Bl/6 mice were instilled with 107 CFU of UPEC. Immediately after each instillation, a single researcher assigned a quality score to the instillation, as defined by specific criteria (boxed text). 24 hr post-infection, bladders wer…

Materials

BD Insyte Autoguard Shielded IV Catheters 24 G, 0.7 mm external diameter, 14 mM long	BD Medical	381811 or 381411*	* catalog number is country dependent
Inoculating loop	Greiner Bio-One	731171
syringes	B Braun	9166017V
Thumb (Adson) forceps	Fine Science Tools	11006-12
insulin syringe	Terumo	BS05M2913
5 ml tubes, polypropylene	Falcon	352063
LB Miller broth	Difco	244620
LB Miller agar	Difco	244520
Cuvettes	Bio-rad	223-9955