A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs

Abstract

Source: Phillips, J. E. et al., Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System. J. Vis. Exp. (2017)

This video demonstrates the generation of drug aerosols and the targeted nose-only delivery of these aerosols to mice using an inhalation unit equipped with an aerosol generator. The mouse is restrained in a nose-only restrainer and connected to the inhalation unit, which delivers the drug directly into the mouse's nasal cavity. Subsequently, the drug passes through the trachea and enters the lungs.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Formulation and Device Selection for the Generation of Pharmaceutical Aerosols NOTE: Formulation and device selection depend on the individual drug's physicochemical properties to be aerosolized. Nebulized liquid aerosols. Dissolve the drug in…

Representative Results

Figure 1: Inhalation unit with dry powder aerosol generator attached. The nebulizer used to generate aerosol from liquid formulations is shown on the right. 1) Wright dust feed, 2) cyclone, 3) syringe pump, 4) jet nebulizer inserted into pre-separator, 5) dilution air mixer. When delivering nebulized aerosols, the dust feed, and cyclone are replaced by the syringe pump, jet nebulizer/pre-sep…

Materials

Nose-only exposure inhalation unit	TSE systems	700100-KNES-040-ss	Custom configurations available
DACO data acquisition system	TSE systems	700400-PRO-C-D/1
MC One Jet Mill	Jetpharma	DEC MicroJet 10
Turbula Mixer	GlenMills Inc	T2F
Micronized Lactose	DFE Pharma	Lactohale 200
Hydraulic press	Specac	GS15011
Cascade impactor filters	Pall Life Sciences	7219	Emfab filter
Absolute filters	Whatman	10370302	5 cm diameter
Real time aerosol monitor Microdust Pro Monitor	Casella	CEL-712
Ipratropium bromide	Spectrum Chemical	I1178	pre-micronized
flexiVent FX1 system	scireq	FV-FXCS