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General Laboratory Techniques
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JoVE Educación científica General Laboratory Techniques
Introduction to Serological Pipettes and Pipettors
  • 00:00Descripción
  • 00:48Serological Pipette Types and Basic Pipette Dispenser Components
  • 03:20Basic Operation of the Serological Pipette
  • 05:05Applications
  • 06:00Summary

Introduction to Serological Pipettes and Pipettors

English

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Descripción

The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions or cell suspensions, transferring liquids between receptacles, or carefully layering reagents of different densities. With careful attention to the level of liquid being aspirated and dispensed, serological pipettes can be useful tools for transferring accurate milliliter volumes of solutions in the lab. This video discusses the way that volume can be read on a serological pipette, how a pipet aid works, and many different applications for using a serological pipette.

Procedimiento

The serological pipette is a nearly ubiquitous laboratory instrument used for transferring milliliter volumes of liquid. Serological pipettes typically have gradations along their sides for measuring the amount of liquid being aspirated or dispensed. These instruments are most commonly used with a pipette dispenser, which facilitates the liquid transfer through the creation of a partial vacuum. The same pipette dispenser can be used with a variety of serological pipette sizes, depending on the amount of volume you…

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No conflicts of interest declared.

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JoVE Science Education Database. JoVE Science Education. Introduction to Serological Pipettes and Pipettors. JoVE, Cambridge, MA, (2023).

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