Moored midwater geodesic structures called Coral Arks provide a modular, scalable, and vertically adjustable research platform that can be used to build, monitor, and perturb coral reef communities in previously inoperative areas, including offshore.
Coral reefs thrive and provide maximal ecosystem services when they support a multi-level trophic structure and grow in favorable water quality conditions that include high light levels, rapid water flow, and low nutrient levels. Poor water quality and other anthropogenic stressors have caused coral mortality in recent decades, leading to trophic downgrading and the loss of biological complexity on many reefs. Solutions to reverse the causes of trophic downgrading remain elusive, in part because efforts to restore reefs are often attempted in the same diminished conditions that caused coral mortality in the first place.
Coral Arks, positively buoyant, midwater structures, are designed to provide improved water quality conditions and supportive cryptic biodiversity for translocated and naturally recruited corals to assemble healthy reef mesocosms for use as long-term research platforms. Autonomous Reef Monitoring Structures (ARMS), passive settlement devices, are used to translocate the cryptic reef biodiversity to the Coral Arks, thereby providing a “boost” to natural recruitment and contributing ecological support to the coral health. We modeled and experimentally tested two designs of Arks to evaluate the drag characteristics of the structures and assess their long-term stability in the midwater based on their response to hydrodynamic forces.
We then installed two designs of Arks structures at two Caribbean reef sites and measured several water quality metrics associated with the Arks environment over time. At deployment and 6 months after, the Coral Arks displayed enhanced metrics of reef function, including higher flow, light, and dissolved oxygen, higher survival of translocated corals, and reduced sedimentation and microbialization relative to nearby seafloor sites at the same depth. This method provides researchers with an adaptable, long-term platform for building reef communities where local water quality conditions can be adjusted by altering deployment parameters such as the depth and site.
Across the globe, coral reef ecosystems are undergoing transitions from high-biodiversity, coral-dominated benthic communities to lower-diversity communities dominated by turf- and fleshy macroalgae1,2,3. Decades of progress in characterizing the mechanisms of coral reef degradation have revealed how links between microbial and macro-organismal communities enhance the pace and severity of these transitions. For example, the overfishing of reefs by human populations initiates a trophic cascade in which excess photosynthetically derived sugars from ungrazed algae shunt energy into the reef microbial communities, thus driving pathogenesis and causing coral decline4,5,6. This trophic downgrading is reinforced by the loss of biodiversity on reefs that results from water quality decline7,8. Mesocosm-level experiments can be used to better understand and mitigate the trophic downgrading of coral reef communities by enhancing biodiversity and improving water quality, but logistical challenges make these studies difficult to implement in situ.
A consequence of trophic downgrading on reefs is the widespread loss of cryptic biodiversity, much of which remains uncharacterized7,9. Corals rely on a diverse suite of cryptic reef organisms ("cryptobiota") that support their health by playing integral roles in predator defense10, cleaning11, grazing competing algae12,13, and the regulation of reef water chemistry14,15. Until recently and due to the methodological limitations of visual surveys, reef cryptobiota have been underrepresented and poorly understood in the context of reef ecology, and they are, thus, rarely considered in efforts to restore or rebuild reefs. In the past decade, the use of standardized settlement units called Autonomous Reef Monitoring Structures (ARMS) combined with high-throughput sequencing approaches has enabled the better collection and characterization of reef cryptobiota16,17. ARMS passively recruit representatives of almost all known coral reef biodiversity and have helped reveal numerous functional roles of cryptic organisms in reef-scale processes9,18,19,20,21,22,23. These settlement units, therefore, provide a mechanism to translocate cryptic reef biota alongside corals in order to assemble more intact reef communities with biologically mediated mechanisms, such as grazing, defense, and enhancement of local water quality, that are essential to maintaining the trophic structure.
Coral-dominated reefs thrive in high-light, low-nutrient, and well-oxygenated environments. Human activities such as urbanization, agriculture, and overfishing have reduced the water quality on many coral reefs by increasing the sediment, nutrients, metals, and other compounds in runoff24,25 and by altering biogeochemical cycling26. In turn, these activities degrade reef communities through smothering, energy depletion, the delivery of pollutants associated with sedimentation27,28, enhancing the growth of macroalgae that compete with corals29, increasing the abundance of microbial pathogens6,30,31, and creating hypoxic zones that kill cryptic invertebrates32,33. These and other "local impacts" are compounded by regional and global changes in ocean conditions, including increasing temperatures and decreasing pH, further worsening the conditions for corals and other reef organisms34,35. At the benthic-water interface, specifically, the respiratory and photosynthetic dynamics of benthic communities cause diel fluctuations in the pH and dissolved oxygen, which become more pronounced on highly degraded reefs, thus creating conditions that benthic invertebrates cannot tolerate32,36,37,38. Providing appropriate water quality conditions is, therefore, essential for assembling functioning reef communities, but this remains challenging because an increasing number of reefs are trapped in various states of degradation.
Many of the challenges faced by corals and foundational cryptic taxa on the benthos may be overcome via relocation to the midwater, defined here as the water column setting between the ocean surface and the seafloor. In the midwater environment, water quality is improved39,40, sedimentation is reduced, and the distance from the seafloor dampens fluctuations in the parameters associated with benthic metabolism. These characteristics are improved further by moving offshore, where land-based anthropogenic impacts, such as terrestrially derived runoff, become increasingly diluted with distance from the coast. Here, we introduce and provide protocols to build, deploy, and monitor Coral Reef Arks, an approach that leverages improved water quality conditions in the midwater and incorporates cryptic biodiversity on anchored, positively buoyant structures for the assembly of coral reef communities.
Coral Reef Arks systems, or "Arks," are comprised of two primary components: (1) a suspended rigid geodesic platform elevated above the benthos and (2) organism-covered or "seeded" ARMS that translocate reef cryptobiota from nearby benthic areas, thereby supplementing the natural recruitment processes to provide the translocated corals with a more diverse and functional reef community. A geodesic structure was selected to maximize the strength and minimize the building material (and, thus, the weight), as well as to create an internal, turbulent flow environment analogous to the reef matrix.
Two designs of Arks were successfully installed at two Caribbean field sites and are currently being used for research into reef community establishment and ecological succession (Figure 1). Coral Arks structures are intended to be long-term research platforms, and as such, a primary focus of this manuscript is to describe protocols to site, install, monitor, and maintain these structures to maximize their stability and longevity in the midwater environment. A combination of modeling and in-water testing was used to evaluate the drag characteristics of the structures and adjust the design to withstand the anticipated hydrodynamic forces. After installation, reef communities were established on the Arks and on nearby benthic control sites at the same depth through a combination of active translocation (corals and seeded ARMS units) and natural recruitment. Water quality conditions, microbial community dynamics, and coral survival on the Arks were documented at several time points throughout the early successional period and compared against the benthic control sites. To date, the conditions associated with the midwater Coral Arks environment have been consistently more favorable for corals and their associated cryptic consortia relative to the neighboring benthic control sites at the same depths. The methods below describe the steps required to replicate the Coral Arks approach, including how to select sites and design and deploy Coral Arks structures. Suggested approaches for monitoring Coral Arks are included in Supplemental File 1.
The representative results presented above demonstrate that Coral Arks provide a habitat and improved water quality conditions for assembling reef communities on stable, in situ research platforms. Arks and seafloor control sites at the same depth displayed consistently different water quality profiles. Higher average current speeds and further distance from the coast reduced sedimentation and turbidity in the midwater environment at the Arks sites (Figure 6B), likely contributing to the lower measured dissolved organic carbon concentrations on the Arks (Figure 6F). Further, these improvements in water clarity resulted in elevated daytime light intensities on the Arks relative to the control sites (Figure 6A). Lower diel fluctuations in dissolved oxygen indicate improved oxygen availability for corals on the Arks compared to the benthos, especially at night (Figure 6G). These metrics have all been associated with improvements in coral survival42, growth43,44,45, and recovery from stress46,47 in past work and may be linked to enhanced survival outcomes of corals translocated to Arks as compared to benthic control sites (Figure 8). The fact that these conditions persist even after the accumulation of substantial biomass through biofouling indicates that natural recruitment processes do not diminish the improved water quality characteristics of the midwater environment. Arks were deployed 3 km offshore of the benthic control sites and likely benefitted from decreased inputs of terrestrially derived sediment, nutrients, and possibly fishing pressures that challenge nearshore sites. Siting Arks in areas with clean water and low human impact (such as offshore) may provide a better setting than heavily impacted coastal zones to propagate reef biodiversity for mesocosm-level experiments.
The preliminary findings also suggested that the midwater Arks experienced less microbialization, a central reef process associated with the degradation of benthic reef habitats4,48. High nutrient inputs and overfishing have been identified as drivers of reef-wide trophic feedback loops in which energetically destabilized microbial communities proliferate, resulting in the respiratory drawdown of metabolically available oxygen and the increased incidence of coral pathogens at the benthos6,49,50,51. The reduced abundance of free viruses on microbialized reefs, which serve as a primary lytic control on microbial community growth, indicate a breakdown in the trophic structure that favors further microbial expansion52. Water column-associated microbes on the Arks were both less abundant (Figure 7B) and physically smaller (Figure 7D) than at the seafloor sites. The Arks also displayed higher virus-to-microbe ratios (Figure 7A), abundance of free viruses (Figure 7C), and dissolved oxygen availability, particularly at night (Figure 6G). Taken together, these findings indicate that the midwater environment displayed less potential for microbialization relative to the seafloor sites. Arks, as mesocosms on which environmental conditions can be altered simply by vertical adjustment in the water column, offer an opportunity to mitigate and further explore the microbial and molecular mechanisms of reef degradation.
Geodesic spheres of two different frequencies were selected for the design of the Coral Arks presented here (Figure 1). Geodesic frequency (1V, 2V, 3V) indicates the number of repeating sub-elements in a geodesic sphere, with higher frequencies corresponding with a higher number of triangular sub-elements. From a structural perspective, geodesic polyhedra distribute mechanical stress throughout the structure, resulting in a high innate strength for their size53,54. These characteristics provide high durability and longevity but come at the cost of higher hydrodynamic drag, which can result in higher loadings on the mooring system. From a habitat perspective, the drag generated by an Ark system represents an indicator of the diffusion of momentum within the structure and, thus, the degree to which the internal ambient flow is reduced. The modeled and experimentally validated results indicate a 40%-70% reduction in the flow speed inside of the "Shell" Arks relative to the surrounding flow field due to the generation of turbulent flow inside the structures (see Section 6 of Supplemental File 1). While the optimal level of internal flow reduction is not clear (and differs with geodesic frequency), areas of reduced flow within the structure are important for creating niche habitats55,56, remineralizing nutrients57,58, and promoting the retention and settlement of larvae59,60. In general, larger and higher frequency geodesic structures, particularly at more exposed installation sites, require anchoring systems with higher holding power and more redundancy incorporated into the structural design.
The results from the field-based measurements of the drag component of tension on the "Shell" Ark mooring system closely matched those results generated from the modeled and experimental towing estimates (Figure 4) and were well within the expected design ranges. These results indicate that the assumptions of the hydrodynamic model are valid and that the model can predict drag forces over the background current ranges. However, while the deviations in the modeled and experimental data were small, the range of flows during the testing period, which were typical of ambient, non-storm flow speeds at the site, did not enable a rigorous validation over the full modeling spectrum. In predicting the design requirements of Coral Arks systems, modeling efforts should be combined with information on storm frequency and exposure at the planned deployment sites to design structures and mooring systems that can survive the anticipated hydrodynamic forces. The modeling work presented here can be used to design Ark systems at other sites with minimal inputs (desired Ark size, frequency, and average current speeds at the deployment site) by providing drag coefficients and maximum expected forces on the mooring and anchoring system.
Arks and ARMS systems are modular and may be built at different scales and with alternative materials than those described here. Although their ultimate longevity has not yet been determined, Coral Arks were designed to have an approximately 10 year life cycle. The material composition of the Arks and ARMS affects the longevity of the structures, the weight of the systems, and, therefore, the required buoyancy to offset the weight and may affect the response of early fouling communities (Supplemental File 1-Figure S7). For example, limestone provides a more natural substrate for biological colonization on the ARMS and is readily and inexpensively sourced on most carbonate reef islands, but it is more fragile and heavier than other materials such as PVC and fiberglass. These factors should be considered against site-specific characteristics to design ARMS, Arks, and mooring systems that best address the desired project outcomes.
The deployment sites for Coral Arks should also be selected based on the intended project goals (i.e., research, mitigation, or restoration). Factors to consider for site selection include the access to materials, reef state or condition, community investment/involvement, resource limitation, institutional support, and permit requirements. Coral Arks may provide opportunities to meet specific needs at sites that (1) contain living coral reefs that are in relatively poor condition and would benefit from restoration activities to enhance the coral recruitment, coral cover, coastal protection, or human food resources; (2) have a need for the translocation of corals to another location, which may occur, for example, when there are legal requirements to move living corals off of debris items slated for removal (at these sites, Coral Arks can be used in collaboration with, or in support of, existing restoration and outplanting efforts to improve translocation outcomes); (3) require research into novel conservation and restoration technologies using Coral Arks to improve the success of local efforts; or (4) have sufficiently distinct local conditions (i.e., different magnitude of anthropogenic impact), meaning standardized mesocosms could yield meaningful comparisons about reef processes and interventions. The specific approaches for monitoring aspects of the Coral Arks ecosystem such as biological growth, diversity, and water chemistry will vary between projects based on the project goals and site-specific variables.A representative outline for the scientific monitoring of Coral Arks conducted to date is provided in Section 5 of Supplemental File 1.
The design of Coral Arks structures can accommodate corals of nearly any species, size, and age and should provide improved conditions relative to those on a disturbed reef benthos. Depending on the growth and calcification rates observed on a given system, the addition of positive buoyancy to the Arks structures may be required to compensate for biological growth and to reduce the risk of sinking. Positively buoyant midwater structures can be weighed using a tension/compression load cell, or strain gauge, to determine if the in-water weight of the community is increasing (Figure 5). Periodic or long-term measurements using the load cell can complement other finer-resolution coral growth metrics to generate a metric of community-level growth/calcification and have been included as a regular maintenance task to determine if the system has sufficient positive buoyancy to compensate for this biological growth over time. In the case that an installed Ark can no longer be monitored or maintained, it could be relocated and/or the buoyancy could be removed to allow the Ark to be firmly attached to the benthos.
The methods described here provide researchers with a versatile toolkit for assembling midwater reef communities that can be sited at locations with improved water quality. By altering the depth or location of the Arks structures, changes in water quality parameters can be experimentally linked to changes in reef community structure and successional trajectories. This design feature allows researchers to exploit the abundant and underutilized space in the midwater environment to assemble and study coral reef mesocosms. The use of seeded ARMS to translocate cryptic biodiversity and deliver a "boost" to the natural recruitment of mobile grazing invertebrates provides a functional solution for reducing algal biofouling and, thus, benthic competition for corals. Using established and standardized sampling structures as components of this system provides added value by enabling the long-term monitoring of cryptic communities on Arks and comparison to datasets generated using ARMS as a global biodiversity census tool.
Coral Arks can serve as a more holistic, integrated, and self-regulating platform for propagating coral and invertebrate biomass that can then be outplanted to nearby degraded reefs and can provide a safe haven for corals to grow and reproduce in improved water quality conditions. As is currently being demonstrated in Puerto Rico, Arks can yield improved survival outcomes for mitigation projects involving the relocation of corals and reef biodiversity from debris items or degraded areas. Arks have relevance in long-term projects as a method to replace habitats for fish populations, test novel conservation strategies, and preserve native reef biodiversity. In the process, Arks provide versatile tools for conducting in situ studies of reef assemblies and ecological succession and may generate novel insights into reef connectivity.
The authors have nothing to disclose.
We thank Mark Vermeij, Kristen Marhaver, and the CARMABI Research Foundation in Curaçao for providing resources, support, and insight for this project. We thank the NAVFAC Atlantic Vieques Restoration Program and the Jacobs Engineering team for their substantial logistical and technical support in installing, maintaining, and monitoring the Coral Arks in Vieques. We are also grateful to Mike Anghera, Toni Luque, Cynthia Silveira, Natascha Varona, Andres Sanchez-Quinto, Lars ter Horst, and Ben Darby for their help and constructive input in the field. This research was funded by a Gordon and Betty Moore Foundation Aquatic Symbiosis Investigator Award to FLR <https://doi.org/10.37807/GBMF9207> and by the Department of Defense Environmental Security Technology Certification Program (RC20-5175).
PVC ARMS | |||
316 Stainless Steel Hex Head Bolt, Partially Threaded, 8" length, 1/4"-20 Thread Size | McMaster Carr | 92186A569 | Bolts for PVC ARMS assembly Per unit: 4x |
316 Stainless Steel Hex Nut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster Carr | 94805A029 | Nuts for PVC ARMS assembly Per unit: 8x |
316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster Carr | 90715A125 | Locknuts for PVC ARMS assembly Per unit: 4x |
316 Stainless Steel Washer for 1/4" Screw Size, 0.281" ID, 0.625" OD | McMaster Carr | 90107A029 | Washers for PVC ARMS assembly Per unit: 8x |
Nylon Unthreaded Spacers – 1/2" Long, 1/2" OD, Black | McMaster Carr | 90176A159 | Nylon spacers for PVC ARMS assembly Per unit: 20x |
PVC Sheet Type 1, 0.25" Thick, Gray | McMaster Carr | 8747K215 | PVC for ARMS stacking plates. See Supplemental File 1-Figure SI 4. Per unit: 9x Refers to drawing: Yes |
PVC Sheet Type 1, 0.5" Thick, Gray | McMaster Carr | 8747K217 | PVC for ARMS baseplates. See Supplemental File 1-Figure SI 1. Per unit: 1x Refers to drawing: Yes |
PVC Sheet Type 1, 0.5" Thick, Gray | McMaster Carr | 8747K217 | PVC for ARMS long cross spacers. See Supplemental File 1-Figure SI 2. Per unit: 4x Refers to drawing: Yes |
PVC Sheet Type 1, 0.5" Thick, Gray | McMaster Carr | 8747K217 | PVC for ARMS short cross spacers. See Supplemental File 1-Figure SI 3. Per unit: 8x Refers to drawing: Yes |
Ratcheting Combination Wrench, 7/16" | McMaster Carr | 5163A15 | Wrenches to secure PVC ARMS hardware Per unit: 2x |
Rebar, 3-ft Lengths, 1/2" Thick | McMaster Carr | 7480N115 | Rebar stakes to secure PVC ARMS to benthos. Mallet required. Per unit: 4x |
Sequentially Numbered Metal Tags | McMaster Carr | 2208N349 | Numbered tags for ARMS ID Per unit: 1x |
Limestone ARMS | |||
DeWalt Wet Tile Saw | Home Depot | D24000S | Cut limestone tile into stackable pieces Per unit: 1x |
Lift Bag, 50 lb Capacity | Amazon | B07GCNGRDR | Lift bag for transport of Limestone ARMS to benthos Per unit: 1x |
Milk Crate, Heavy Duty, 13" x 19" x 11" | Amazon | B06XGBDJMD | Crate for transport of Limestone ARMS to benthos Per unit: 1x |
Natural Limestone or Travertine Tile (Unfilled) – 12" x 12" | Bedrosians Tile & Stone | TRVSIENA1212T | Base material for Limestone ARMS layers and stacking pieces. See Supplemental File 1-Figure SI 7 and Figure SI 8. Per unit: 10x Refers to drawing: Yes |
PC-11 Epoxy Adhesive Paste, Two-Part Marine Grade | Amazon | B008DZ1864 | Two-part epoxy for Limestone ARMS assembly |
Shell Ark | |||
Downline: 1" Nylon, 6' length thimble-to-thimble with stainless sailmaker thimble at top, heavy duty galvanized thimble at bottom | West Marine | Custom | Nylon mooring line for attaching Ark mooring bridle to anchor system. Per unit: 1 |
Main structure: 105-B Epoxy | West Marine (made by West System) | 318352 | Epoxy to seal foam in struts. |
Main structure: 205-B Hardener | West Marine (made by West System) | 318378 | Epoxy to seal foam in struts. |
Mooring bridle: 3-1/8" X 2" small diamond base padeye with 7/8" bail | West Marine (Made by Harken) | 130560 | Padeyes for attaching mooring system to Ark base. Per unit: 5 |
Main structure: 3/4" H-80 Divinycell Closed-Cell Foam, Plain Sheet 48" x 96" | Fiberglass Supply | L18-1110 | Buoyant foam for struts. Cut foam into 1.5" wide strips, 15.5" long for S1 struts and 19" long for S2 struts, add to struts. Per unit: 120 |
Downline: 3/4" Stainless Masterlink | Lift-It (Made by Suncor) | S0652-0020 | Masterlink, connects top of swivel to lower portion of 5-point mooring bridle. Per unit: 1 |
Mooring bridle: 3/8" Stainless Long D Shackles with Captive Self-Locking Pin | West Marine (Made by Wichard) | 116293 | High-strength shackles to connect pad eyes to mooring system. Per unit: 5 |
Main structure: 316 SS, Pan Head Phillips Screw, 1/4-20, 3" Long | McMaster Carr | 91735A385 | Bolts to attach hull anodes to stainless struts Per unit: 2 |
ARMS attachments: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/2"-13 Thread Size | McMaster | 90715A165 | Locknuts for attaching ARMS to ARMS mounting baseplates (8 per unit) Per unit: 80 |
ARMS Baseplates: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster | 90715A125 | Locknuts for ARMS mounting baseplates (struts and Stars) Per unit: 600 |
Coral plate baseplates: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster | 90715A125 | Locknuts for attaching coral plate baseplates to struts Per unit: 600 |
Coral plate attach: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster | 90715A125 | Locknuts to attach coral plates to baseplates Per unit: 80 |
Mooring bridle: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 1/4"-20 Thread Size | McMaster | 90715A125 | Padeye locknuts for attaching pad eyes to struts. Per unit: 20 |
Main structure: 316 Stainless Steel Nylon-Insert Locknut, Super-Corrosion-Resistant, 10-32 Thread Size | McMaster | 90715A115 | Locknuts for star-strut connections Per unit: 475 |
Main structure: 316 Stainless Steel Pan Head Phillips Screw, 10-32 Thread, 2-1/2" Long | McMaster | 91735A368 | Bolts for star-strut connections Per unit: 475 |
Mooring bridle: 316 Stainless Steel Phillips Flat Head Screws, 1/4"-20 Thread Size, 2-3/4" Long | McMaster | 91500A341 | Padeye bolts for attaching pad eyes to struts. Per unit: 15 |
ARMS Baseplates: 316 Stainless Steel Phillips Flat Head Screws, 1/4"-20 Thread Size, 3" Long | McMaster | 91500A554 | Bolts for attaching ARMS mounting baseplates to Stars Per unit: 475 |
Mooring bridle: 316 Stainless Steel Phillips Flat Head Screws, 1/4"-20 Thread Size, 3" Long | McMaster | 91500A554 | Padeye bolts for attaching pad eyes through struts & Stars. Per unit: 5 |
Mooring bridle: 316 Stainless Steel Screw-Pin Shackle – for Lifting, 1/2" Thick | McMaster | 3583T15 | Shackles to connect lower bridle thimbles to small links on Masterlink. Per unit: 5 |
ARMS attachments: 316 Stainless Steel Split Lock Washer for 1/2" Screw Size, 0.512" ID, 0.869" OD | McMaster | 92147A033 | Lock washers for attaching ARMS to ARMS mounting baseplates (4 per unit) Per unit: 40 |
ARMS attachments: 316 Stainless Steel Washer for 1/2" Screw Size, 0.531" ID, 1.25" OD | McMaster | 90107A033 | Backing washers for attaching ARMS to ARMS mounting baseplates (4 per unit) Per unit: 40 |
ARMS Baseplates: 316 Stainless Steel Washer for 1/4" Screw Size, 0.281" ID, 0.625" OD | McMaster | 90107A029 | Washers for attaching ARMS mounting baseplates to struts Per unit: 40 |
Coral plate baseplates: 316 Stainless Steel Washer for 1/4" Screw Size, 0.281" ID, 0.625" OD | McMaster | 90107A029 | Washers for attaching coral plate baseplates to struts Per unit: 40 |
Coral plate attach: 316 Stainless Steel Washer for 1/4" Screw Size, 0.281" ID, 0.625" OD | McMaster | 90107A029 | Washers to attach coral plates to baseplates Per unit: 160 |
Main structure: 316 Stainless Steel Washer for Number 10 Screw Size, 0.203" ID, 0.438" OD | McMaster | 90107A011 | Washers for star-strut connections Per unit: 475 |
Buoyancy: 316 Stainless Steel Washer, 1" Screw Size, 2" OD | McMaster | 90107A038 | Large washers for central rod (2 per float) Per unit: 22 |
ARMS attachments: 316 Stainless Steel Washer, Oversized, 1/2" Screw, 1.5" OD, 0.052"- 0.072" Thickness | McMaster | 91525A145 | Oversized washers for attaching ARMS to ARMS mounting baseplates (4 per unit) Per unit: 40 |
Coral plates: 3M Marine Adhesive Sealant – Fast Cure 5200 | McMaster | 67015A44 | Adhesive to glue limestone tiles to PVC coral baseplates. Drill out corners with masonry bit. |
Buoyancy: 3M Marine Adhesive Sealant – Fast Cure 5200 | McMaster | 67015A44 | Adhesive for securing fiberglass threaded rods into trawl floats Per unit: 2 |
Mooring bridle: 5/8" Dyneema with Stainless Sailmakers Thimbles at Top and Bottom | West Marine | Custom | 5-leg mooring bridle for attaching Ark to downline. Per unit: 5 |
Downline: Clevis-to-Clevis Swivel – Not for Lifting, 316 Stainless Steel, 6-7/32" Long | McMaster | 37405T29 | Swivel, bottom connects to top of downline, top connects to large link in Masterlink. Per unit: 1 |
Buoyancy: Fiberglass Hex Nut, 1"-8 Thread Size | McMaster | 91395A038 | Fiberglass hex nuts for securing fiberglass threaded rods into trawl floats Per unit: 30 |
Buoyancy: Fiberglass Threaded Rod, 1"-8 Thread Size, 8 Feet Long | McMaster | 91315A238 | Fiberglass threaded rod to attach float to Ark. See Supplemental File 1-Figure SI 16. Per unit: 10 Refers to drawing: Yes |
Anchor system: Galvanized Alloy Steel Shackle with Screw Pin – for Lifting, 1/2" Thick | McMaster | 3663T42 | Middle shackle from chain to pear link. Per unit: 3 |
Anchor system: Galvanized Alloy Steel Shackle with Screw Pin – for Lifting, 3/4" Thick | McMaster | 3663T44 | Upper large shackle to connect pear link to lower downline thimble. Per unit: 1 |
Anchor system: Galvanized Alloy Steel Shackle with Screw Pin – for Lifting, 3/4" Thick | McMaster | 3663T44 | Anchor shackle. Per unit: 3 |
Anchor system: Galvanized Alloy Steel Shackle with Screw Pin – for Lifting, 3/8" Thick | McMaster | 3663T51 | Shackle to connect chain to upper middle shackle. Per unit: 3 |
Anchor system: Galvanized Alloy Steel Shackle with Screw Pin – for Lifting, 3/8" Thick | McMaster | 3663T51 | Lower small shackle to connect chain and anchor shackle. Per unit: 3 |
Install & Tools: HARKEN–57mm Carbo Air® Triple Block | West Marine | 200076 | Top of block and tackle Per unit: 1 |
Install & Tools: HARKEN–57mm Carbo Air® Triple Block with Becket and Cam | West Marine | 1171644 | Base of block and tackle Per unit: 1 |
ARMS Baseplates: Heat-Shrink Tubing, 0.50" ID Before Shrinking | McMaster | 7856K47 | Heatshrink for non-slip. Cut into 1.5" lengths, slide over a SS u-bolt bracket and use heat gun to tighten onto bracket. Per unit: 20 |
Coral plate baseplates: Heat-Shrink Tubing, 0.50" ID Before Shrinking | McMaster | 7856K47 | Heatshrink for non-slip. Cut into 1.5" lengths, slide over a SS u-bolt bracket and use heat gun to tighten onto bracket. Per unit: 40 |
Buoyancy: Heatshrink for covering threaded rods before mounting in floats, 14" sections | McMaster | 7856K66 | Heatshrink for non-slip. Cut into 14" lengths. Slide onto fiberglass rods with 1" exposed on one end and 2-1/4" exposed on the other. Use heat gun to shrink until snug. Per unit: 11 |
Anchor system: High-Strength Grade 40/43 Chain-Not for Lifting, Galvanized Steel, 5/16 Trade Size | McMaster | 3588T23 | Chain to connect anchors and downline. Per unit: 3 |
Install & Tools: LOW-STRETCH ROPE, 7/16" DIAMETER | McMaster | 3789T25 | Rope for block and tackle Per unit: 250 |
ARMS Baseplates: Marine-Grade Moisture-Resistant HDPE, 48" x 48", 1/2" Thick | McMaster | 9785T82 | Sheeting for ARMS mounting baseplates. See Supplemental File 1-Figure SI 13. Per unit: 10 Refers to drawing: Yes |
Coral plate baseplates: Marine-Grade Moisture-Resistant HDPE, 48" x 48", 1/2" Thick | McMaster | 9785T82 | Sheeting for coral plate baseplates. See Supplemental File 1-Figure SI 14. Per unit: 20 Refers to drawing: Yes |
Mooring bridle: Martyr Collar Anode Zinc 3/4" x 2 1/8" x 2 1/8" | West Marine | 5538715 | Sacrificial anodes for Masterlinks on mooring lines Per unit: 2 |
Main structure: Martyr Hull Anode Zinc 6 1/4" x 2 3/4" x 5/8" | West Marine | 484998 | Sacrificial anodes for stainless struts at Ark base Per unit: 3 |
ARMS Baseplates: Mounting Plate for 1/4"-20 Thread Size, 2" ID 304 Stainless Steel U-Bolt | McMaster | 8896T156 | Bracket plate w/heatshrink, for attaching ARMS mounting baseplates to struts Per unit: 6 |
Coral plate baseplates: Mounting Plate for 1/4"-20 Thread Size, 2" ID 304 Stainless Steel U-Bolt | McMaster | 8896T156 | Bracket plate w/heatshrink, for attaching coral plate baseplates to struts Per unit: 40 |
Main structure: N1 Stars, 316 SS, 5mm Thick Connectors for DIY VikingDome F2 Sphere, modified | Viking Dome | ICO2-AISI | N1 Stars modified for central rod. Machine/weld connections to insert top and bottom of unthreaded fiberglass structural rod. See Supplemental File 1-Figure SI 10. Per unit: 2 |
Main structure: N1 Stars, 316 SS, 5mm Thick Connectors for DIY VikingDome F2 Sphere, unmodified | Viking Dome | ICO2-AISI | Unmodified N1 Stars for Ark assembly. See Supplemental File 1-Figure SI 10 Per unit: 10 Refers to drawing: Yes |
Main structure: N2 Stars, 316 SS, 5mm Thick Connectors for DIY VikingDome F2 Sphere, modified | Viking Dome | ICO2-AISI | N2 Stars modified for floats. Drill larger center hole to accommodate 1" threaded fiberglass rod. Per unit: 10 |
Main structure: N2 Stars, 316 SS, 5mm Thick Connectors for DIY VikingDome F2 Sphere, modified | Viking Dome | ICO2-AISI | N2 Stars modified for pad eyes. Drill larger bolt hole (bit – 1/4") on outer hole of one arm for Padeye connector. Per unit: 5 |
Main structure: N2 Stars, 316 SS, 5mm Thick Connectors for DIY VikingDome F2 Sphere, unmodified | Viking Dome | ICO2-AISI | Unmodified N2 Stars for Ark assembly Per unit: 15 |
Anchor system: Pear-Shaped Link – Not for Lifting, Galvanized Steel, 3/4" Thick | McMaster | 3567T34 | Link to connect 3x 1/2" shackles to upper large shackle. Per unit: 1 |
Install & Tools: Phillips Screwdriver, Size No. 2 | McMaster Carr | 5682A28 | Tighten down locknuts on star-strut bolts Per unit: 1 |
Coral plates: PVC Sheet Type 1, Gray, 48" x 48", 1/4" Thick | McMaster | 8747K194 | PVC baseplates for coral plates. See Supplemental File 1-Figure SI 4. Per unit: 20 Refers to drawing: Yes |
Install & Tools: Ratcheting Combination Wrench, 3/4" | McMaster Carr | 5163A21 | Attach ARMS to ARMS mounting baseplates Per unit: 2 |
Install & Tools: Ratcheting Combination Wrench, 3/8" | McMaster Carr | 5163A14 | Tighten down locknuts on star-strut bolts Per unit: 2 |
Install & Tools: Ratcheting Combination Wrench, 7/16" | McMaster Carr | 5163A15 | Attach coral plates to coral plate baseplates Per unit: 2 |
Install & Tools: Round Bend-and-Stay Multipurpose Stainless Steel Wire, 0.012" diameter, 645 feet | McMaster | 9882K35 | Wire for mousing stainless shackles Per unit: 1 |
Main structure: S1 Struts – Structural FRP Fiberglass Square Tube, 2" Wide x 2" High Outside, 1/4" Wall Thickness | McMaster | 8548K34 | Fiberglass S1 Struts. Cut to 20.905" long (531 mm), drill bolt holes (bit – 7/32"), fill w/ divinycell foam & epoxy. See Supplemental File 1-Figure SI 9 Per unit: 55 Refers to drawing: Yes |
Main structure: S1 Struts (SS) – Corrosion-Resistant 316/316L Stainless Steel Rectangular Tube, 0.12" Wall Thickness, 2" x 2" Outside | McMaster | 2937K17 | Stainless S1 Struts. Cut to 20.905" long (531 mm), drill bolt holes (bit – 1/4"). See Supplemental File 1-Figure SI 9. Per unit: 5 Refers to drawing: Yes |
Main structure: S2 Struts – Structural FRP Fiberglass Square Tube, 2" Wide x 2" High Outside, 1/4" Wall Thickness | McMaster | 8548K34 | Fiberglass S2 Struts. Cut to 24.331" long (618 mm), drill bolt holes (bit – 7/32"), fill w/ divinycell foam & epoxy. See Supplemental File 1-Figure SI 9. Per unit: 60 Refers to drawing: Yes |
Anchor system: Skrew SK2500 | Spade Anchor USA | SK2500 | Two-plate sand screw anchors Per unit: 3 |
Coral plates: Stainless Steel Washers for 1/4" Screw Size, 0.281" ID, 0.625" OD | McMaster | 90107A029 | Numbered tags for coral plates. Stamp SS washers with numbered stamps and glue to coral plate for later ID. Per unit: 100 |
Main structure: Structural FRP Fiberglass Rod, 10 Feet Long, 1" Diameter | McMaster | 8543K26 | Central fiberglass rod, cut to Ark diameter Per unit: 1 |
ARMS attachments: Super-Corrosion-Resistant 316 Stainless Steel Hex Head Screw, 1/2"-13 Thread Size, 1-3/4" Long | McMaster | 93190A718 | Bolts for attaching ARMS to ARMS mounting baseplates (4 per unit) Per unit: 40 |
Coral plate attach: Super-Corrosion-Resistant 316 Stainless Steel Hex Head Screw, 1/4"-20 Thread Size, 2" Long, Fully Threaded | McMaster | 93190A550 | Bolts to attach coral plates to baseplates Per unit: 80 |
ARMS Baseplates: Super-Corrosion-Resistant 316 Stainless Steel Hex Head Screw, 1/4"-20 Thread Size, 3-1/2" Long | McMaster | 92186A556 | Bolts for attaching ARMS mounting baseplates to struts Per unit: 40 |
Coral plate baseplates: Super-Corrosion-Resistant 316 Stainless Steel Hex Head Screw, 1/4"-20 Thread Size, 3" Long, Partially Threaded | McMaster | 92186A554 | Bolts for attaching coral plate baseplates to struts Per unit: 160 |
Buoyancy: TFLOAT 14" CENTERHOLE OR 437FM, modified | Seattle Marine | YUN12B-8 | 14" trawl floats for mounting to Stars. Slide fiberglass rod with heat shrink through trawl float. Add stainless washer and fiberglass hex nut on both sides. Seal washers with 3M 5200. Tighten nuts down. See Supplemental File 1-Figure SI 16. Per unit: 11 Refers to drawing: Yes |
Buoyancy: TFLOAT 14" CENTERHOLE OR 437FM, unmodified | Seattle Marine | YUN12B-8 | 14" trawl float Per unit: 2 |
ARMS Baseplates: Thick-Wall Dark Gray PVC Pipe for Water, Unthreaded, 1/4 Pipe Size, 5 Feet Long | McMaster | 48855K41 | Star standoffs for attaching ARMS mounting baseplates to Stars. Cut to 1.75" long sections. Per unit: 40 |
Coral plates: Unfilled, Natural Travertine Flooring Tile, 16" x 16" | Home Depot | 304540080 | Limestone tiles for coral plates. Cut to 9" x 9" tiles using wet tile saw. Per unit: 20 |
Buoyancy: Vibration-Damping Routing Clamp, Weld mount, Polypropylene with Stainless Steel Plates, 1" ID | McMaster | 3015T47 | Attachment for central rod and float Per unit: 1 |
Buoyancy: Water- and Steam-Resistant Fiberglass Washer for 1" Screw Size, 1.015" ID, 1.755" OD | McMaster | 93493A110 | Fiberglass washers for securing fiberglass threaded rods into trawl floats Per unit: 20 |
Install & Tools: Zinc-Galvanized Steel Wire, 0.014" diameter, 475 feet long | McMaster | 8872K19 | Wire for mousing galvanized shackles Per unit: 1 |
Two Platform Ark | |||
Downline: 1" Nylon, 15' length thimble-to-thimble with SS Sailmaker Thimble spliced at top, galvanized thimble spliced at bottom | West Marine | Custom | Runs from bottom of swivel shackle (SS) to top of anchor system (galvanized) Per unit: 1x |
Downline: 1/2" Spectra Rope with SS316 Sailmakers Thimbles Spliced at Top and Bottom | West Marine | Custom | Runs from bottom of Ark to top of swivel shackle. Per unit: 2x |
Buoyancy: 1/2" Spectra Rope with SS316 Sailmakers Thimbles Spliced at Top and Bottom | West Marine | Custom | Connects mooring buoy to top eye on Ark Per unit: 2x |
Main structure: 3/8 x 36 Inch SS Thimble Eye Swages and 5/8 Jaw-Jaw Turnbuckle Cable Assembly | Pacific Rigging & Loft | Custom | Custom rigging system with turnbuckle, 3/8" SS wire rope swaged into PVC end caps Per unit: 1x |
Main structure: 304 SS U-Bolt with Mounting Plate, 1/4"-20, 2" ID | McMaster Carr | 8896T123 | For joining fiberglass platforms using I-beams Per unit: 10x |
Main structure: 316 SS Hex Nut, 1/4"-20 | McMaster Carr | 94804A029 | For locking struts in hubs Per unit: 120x |
Main structure: 316 SS Nylon-Insert Locknut, 1/4"-20 | McMaster Carr | 90715A125 | For locking struts in hubs Per unit: 240x |
Main structure: 316 SS Pan Head Phillips Screw, 1/4"-20 Thread, 2.5" Long | McMaster Carr | 91735A384 | For locking struts in hubs Per unit: 120x |
Downline: 316 SS Safety-Pin Shackle, 1/2" Thick | McMaster Carr | 3860T25 | Connect Ark bottom eye to 1/2" Spectra rope. Per unit: 1x |
Buoyancy: 316 SS Safety-Pin Shackle, 1/2" Thick | McMaster Carr | 3860T25 | Connects bottom of 1/2" rope to top Ark eye Per unit: 2x |
Buoyancy: 316 SS Safety-Pin Shackle, 7/16" Thick | McMaster Carr | 3860T24 | Connects mooring buoy to 1/2" rope Per unit: 2x |
Install & Tools: Arbor with 7/16" Hex for 1-1/2" Diameter Hole Saw | McMaster Carr | 4066A63 | Drill holes in 6" PVC (Hubs) Per unit: 1x |
Main structure: Clamping U-bolt, 304 SS, 1/4"-20 Thread Size, 9/16" ID | McMaster Carr | 3042T149 | For clamping SS wire rope at Ark vertices Per unit: 15x |
Downline: Clevis-to-Clevis Swivel, 316 SS, 5-7/16" Long | McMaster Carr | 37405T28 | Swivel shackle between 1/2" spectra rope and 1" nylon downline Per unit: 1x |
Main structure: Corrosion-Resistant Wire Rope, 316 SS, 1/8" Thick | McMaster Carr | 8908T44 | String through assembled Ark and clamp at vertices Per unit: 250ft |
Main structure: Fiberglass Molded Grating, Square Grid, 1" Grid Height, 1-1/2" x 1-1/2" Square Grid, Grit Surface, 70% Open Area | McNichols | MS-S-100 | Cut to half pentagon shape, mirror images. See Figure S23. Per unit: 2x Refers to drawing: Yes |
Anchor system: Galvanized Alloy Steel Screw-Pin Shackle, 1/2" Thick | McMaster Carr | 3663T42 | Connects base of 1" nylon downline to anchor chain Per unit: 1x |
Anchor system: Galvanized Alloy Steel Screw-Pin Shackle, 3/8" Thick | McMaster Carr | 3663T51 | Connects anchor chain together Per unit: 1x |
Anchor system: Grade 30 Chain, Galvanized Steel, 1/4 Trade Size | McMaster Carr | 3592T45 | Anchor chain |
Install & Tools: HARKEN–57 mm Carbo Air Triple Block | West Marine | 200076 | Top of block and tackle Per unit: 1x |
Install & Tools: HARKEN–57 mm Carbo Air Triple Block with Becket and Cam | West Marine | 1171644 | Base of block and tackle Per unit: 1x |
Install & Tools: Hole Saw, 1-15/16" Cutting Depth, 1-1/2" Diameter | McMaster Carr | 4066A27 | Drill holes in 6" PVC (Hubs) Per unit: 1x |
Install & Tools: Low Pressure Inflator Nozzle | Amazon (Made by Trident) | B00KAI940E | Inflate mooring buoys underwater Per unit: 1x |
Install & Tools: LOW-STRETCH ROPE, 7/16" DIAMETER | McMaster | 3789T25 | Rope for block and tackle Per unit: 100ft |
Main structure: Nylon Cable Ties, UV Resistant Heavy Duty, 19" long, 250 lb strength | CableTiesAndMore | CT19BK | Use to secure platforms to Ark framework Per unit: 30x |
Install & Tools: Phillips Screwdriver, Size No. 3 | McMaster Carr | 5682A29 | For locking struts in hubs Per unit: 1x |
Buoyancy: Polyform Buoy, A-5 Series All-Purpose Buoy, 27" | West Marine (Made by PolyformUS) | 11630142 | Mooring buoy for buoyancy. Per unit: 2x |
Main structure: PVC Pipe, Schedule 80, 1" diameter | McMaster Carr | 48855K13 | Struts. Cut to 1.2 m (4 ft) lengths, drill to accommodate bolts Per unit: 30x |
Main structure: PVC Pipe, Schedule 80, 6" diameter | McMaster Carr | 48855K42 | Hubs. Cut into 4" lengths, drill 5 holes symmetrically around midline using 1-1/2" hole saw. See Supplemental File 1-Figure S22. Per unit: 12x Refers to drawing: Yes |
Main structure: PVC Thick Wall Pipe Fitting, End Cap, Schedule 80, 6 " diameter, Female | PRMFiltration (Made by ERA) | PVC80CAP600X | End caps for top and bottom of Ark. Cut off bottom 2 inches. Per unit: 2x |
Install & Tools: Ratcheting Combination Wrench, 7/16" | McMaster Carr | 5163A15 | For locking struts in hubs Per unit: 1x |
Install & Tools: Ratcheting PVC Cutter, 1-1/4" | McMaster Carr | 8336A11 | Cut 1" PVC into struts Per unit: 1x |
Main structure: Ring, 18-8 SS, for 5/32 Chain Trade Size, 3/4" Inside Length | McMaster Carr | 3769T71 | Substitute for 1/2" SS wire rope clamps. Per unit: 12x |
Install & Tools: Round Bend-and-Stay Multipurpose Stainless Steel Wire, 0.012" diameter, 645 feet | McMaster | 9882K35 | Wire for mousing stainless shackles Per unit: 1 |
Main structure: Structural FRP Fiberglass I-Beam, 1/4" Wall Thickness, 1-1/2" Wide x 3" High, 5 ft long | McMaster Carr | 9468T41 | Cut to 5 1-ft long sections. Per unit: 1x |
Install & Tools: Underwater Lift Bag, 220 lbs Lift Capacity | Subsalve Commercial | C-200 | Transport Ark to deployment site Per unit: 1x |
Install & Tools: Zinc-Galvanized Steel Wire, 0.014" diameter, 475 feet long | McMaster | 8872K19 | Wire for mousing galvanized shackles Per unit: 1x |
Strain Gauge | |||
316 Stainless Steel Eyebolt, for Lifting, M16 x 2 Thread Size, 27 mm Thread Length | McMaster Carr | 3130T14 | For strain gauge eyebolts Per unit: 2x |
Bridge101A Data Logger, 30 mV | MadgeTech | Bridge101A-30 | Collect voltage data from load cell. Per unit: 1x |
Chemical-Resistant PVC Rod, 2" Diameter | McMaster Carr | 8745K26 | For datalogger housing endcap. See Supplemental File 1-Figure S32. Per unit: 1x Refers to drawing: Yes |
Clamping U-Bolt, 304 SS, 5/16"-18 Thread Size, 1-3/8" ID | McMaster Carr | 3042T154 | For attachment of datalogger housing to strain gauge. Per unit: 1x |
Dow Corning Molykote 44 Medium Grease Lubricant | Amazon (Made by Dow Corning) | B001VY1EL8 | For mating male and female underwater connectors. Per unit: 1x |
STA-8 Stainless Steel S Type Tension and Compression Load Cell | LCM Systems | STA-8-1T-SUB | Load cell instrument for assessment of in-water weight. Per unit: 1x |
Standard-Wall Clear Blue Rigid PVC Pipe for Water, Unthreaded, 1-1/2 Pipe Size, 2 ft | McMaster Carr | 49035K47 | For datalogger housing. See Supplemental File 1-Figure S31. Per unit: 1x Refers to drawing: Yes |
Standard-Wall PVC Pipe Fitting for Water, Cap, White, 1-1/2 Pipe Size Socket Female | McMaster Carr | 4880K55 | For datalogger housing. Per unit: 2x |
Structural FRP Fiberglass Sheet, 12" Wide x 12" Long, 3/16" Thick | McMaster Carr | 8537K24 | For attachment of datalogger housing to strain gauge. Per unit: 1x |
SubConn Micro Circular Connector, Female, 4-port | McCartney (Made by SubConn) | MCBH4F | Install into machined housing endcap. Per unit: 1x |
SubConn Micro Circular Connector, Male, 4-contact | McCartney (Made by SubConn) | MCIL4M | Splice to load cell wiring and waterproof connection. Per unit: 1x |
Threadlocker, Loctite 262, 0.34 FL. oz Bottle | McMaster Carr | 91458A170 | For strain gauge eyebolts Per unit: 1x |
Vibration-Damping Routing Clamp, Weld-Mount, Polypropylene with Zinc-Plated Steel Top Plate, 1-7/8" ID | McMaster Carr | 3015T39 | For attachment of datalogger housing to strain gauge. Per unit: 1x |