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Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
 

Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

Article DOI: 10.3791/1714-v • 05:30 min • February 11th, 2010
February 11th, 2010 •
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1Department of Molecular and Cell Biology, University of Connecticut

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This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.

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Hemocytes Hawaiian Bobtail Squid Euprymna Scolopes Adherence Symbiotic Bacteria Non-symbiotic Bacteria Immune System Molecular Signaling Beneficial Bacteria Co-evolution Eukaryotes Microbiota Model System Animal Development Macrophage-like Hemocytes Innate Immune System Establishment Maintenance Protocol Anesthetized Hemolymph Collection Cephalic Blood Vessel Chambered Glass Coverslips Green Fluorescent Protein-labeled Symbiotic Vibrio Fischeri Non-symbiotic Vibrio Harveyi Fluorescent Dye Cell Tracker Orange Visualized Fluorescent Microscopy
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