Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Differentiation of Embryonic Stem Cells into…
JoVE Journal
Biology

Esto contenido es Open Access.

JoVE Journal Biology
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
 

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Article DOI: 10.3791/1960-v • 08:11 min • May 19th, 2010
May 19th, 2010 •
1, 1, 1
1Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine, School of Medicine, University of California, Davis

Capítulos

Resumen
Automatic Translation

We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

Tags

Embryonic Stem Cells Oligodendrocyte Precursors Myelinating Cells Central Nervous System Regenerative Cell Therapy Demyelinating Diseases Transplantation Transcription Factor Olig2 Proteoglycan NG2 GFP-Olig2 Mouse Embryonic Stem Cell Reporter Line Differentiation Small Molecules Retinoic Acid Sonic Hedgehog Agonist Purmorphamine Electrophysiological Property NaV1.2 Subunit
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter