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Neuroscience

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JoVE Journal Neuroscience
Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
 

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

Article DOI: 10.3791/2412-v • 12:53 min • January 14th, 2011
January 14th, 2011 •
1, 2, 1
1Institute of Healthy Ageing, and GEE, University College London - UCL, 2School of Biosciences, University of Kent

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The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber interneurons.

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Electrophysiological Recordings Giant Fiber Pathway D. Melanogaster Startle Response Neuronal Circuit Giant Fiber System (GFS) Giant Fiber Interneurons Axons Thoracic Ganglion Cervical Connective Mesothoracic Neuromere (T2) Ventral Ganglia Electro-chemical Synapses Medial Dendrite Ipsilateral Motorneuron (TTMn) Tergotrochanteral Muscle (TTM) Mesothoracic Femur/leg Peripherally Synapsing Interneuron (PSI) Cholinergic Synapses Motorneurons (DLMns) Dorsal Longitudinal Muscles (DLMs) Wing Depressors Dorsovental Muscles (DVMs) Indirect Flight Muscles
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