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Isolatie & Karakterisering van Hoechst Lage CD45 Negatief Muis Lung Mesenchymale stamcellen
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
DOI:

16:55 min

October 26, 2011

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Capítulos

  • 00:05Título
  • 01:49Lung Isolation
  • 04:05Preparation of a Single Cell Suspension from Lung Tissue
  • 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
  • 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
  • 14:49Representative Analysis of Isolated MSCs
  • 16:08Conclusion

Summary

Traducción Automática

In dit artikel tonen we de isolatie van murine inwoner long mesenchymale stamcellen (MSC long), hun expansie, karakterisering en analyse van immunomodulerende eigenschappen.

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