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可视化和肝脏微循环血流量和耗氧量分析：急性肝炎模型中的应用

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Medicina
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JoVE Revista Medicina

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

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可视化和肝脏微循环血流量和耗氧量分析：急性肝炎模型中的应用

DOI:

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10:40 min

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August 04, 2012

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Kosuke Tsukada, Makoto Suematsu³

¹Department of Applied Physics and Physico-Informatics,Keio University, ²Department of Biochemistry, School of Medicine,Keio University, ³Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project,Japan Science and Technology Agency (JST)

Capítulos

00:05Título
02:44Labeling Erythrocytes with Fluorescein Isothiocyanate Isomer (FITC)
04:11Preparation of Oxygen-sensitive Dye
04:54Animal Preparation
05:58Blood Flow Visualization
06:15pO2 Measurement
08:04Hepatitis Model
08:31Representative Results
10:12Conclusion

Summary

Traducción Automática

光学系统的开发，可视化与FITC标记的红细胞肝脏微循环和氧气在微血管局部压力测量激光辅助磷光。这种方法可以用来研究生理和病理机制，通过分析微血管结构，直径，血流速度和氧张力。

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