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JoVE Journal Neuroscience
In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
 

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Article DOI: 10.3791/4384-v • 12:42 min • October 16th, 2012
October 16th, 2012 •
1, 1
1Institute of Molecular Life Sciences, University of Zurich

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A method by which gene expression in the neural tube can be downregulated in a cell type-specific, traceable manner is described. We demonstrate how in ovo electroporation of microRNA-based plasmids that elicit spatiotemporally controlled RNA interference can be used to investigate commissural axon guidance in the developing neural tube.

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Ovo Electroporation MiRNA-based Plasmids Developing Neural Tube Phenotypes DiI Injection Open-book Preparations Commissural DI1 Neurons Axon Guidance Dorsal Spinal Cord Floorplate Rostral Turn Brain Projection Attractive And Repulsive Guidance Cues Gene Knockdown In Vivo Chicken Neural Tube Cell Type-specific Promoters/enhancers Fluorescent Protein Marker MiR30-RNAi
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