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JoVE Journal Neuroscience
Calcium Phosphate Transfection of Primary Hippocampal Neurons
 

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Article DOI: 10.3791/50808-v • 08:22 min • November 12th, 2013
November 12th, 2013 •
*1, *1, 1, 1, 1
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University
* These authors contributed equally

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Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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Calcium Phosphate Precipitation Transfection Primary Hippocampal Neurons Cultured Cells Hard-to-transfect Cells Protocol Astroglial Cells
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