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AFM-gebaseerde kaart brengen van de elastische eigenschappen van celwanden: op weefsel-, cellulair en Subcellulaire Resoluties
JoVE Revista
Biología
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JoVE Revista Biología
AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
DOI:

10:26 min

July 24, 2014

Capítulos

  • 00:05Título
  • 01:17Preparing Glass Slides for Mounting Samples
  • 02:06Dissecting and Mounting Samples
  • 04:54AFM Preparation and Sensitivity Calibration
  • 06:44Data Acquisition: Apparent Young’s Modulus Cartography
  • 07:49Data Analysis: Apparent Young’s Modulus Calculations
  • 08:42Results: Mechanical Properties of Plant Tissues Measured by AFM Micro/Nano-indentations
  • 10:06Conclusion

Summary

Traducción Automática

We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.

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