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Journal / Bioengineering / Harmonic Nanoparticles for Regenerative Research
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Bioengineering

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JoVE Journal Bioengineering
Harmonic Nanoparticles for Regenerative Research
 

Harmonic Nanoparticles for Regenerative Research

Article DOI: 10.3791/51333-v • 09:23 min • May 1st, 2014
May 1st, 2014 •
1, 2, 1, 3, 4, 5, 6, 4, 2, 2, 1
1Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 2Physics Department, GAP-Biophotonics, University of Geneva, 3Laboratoire d'Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l'Ingénieur, École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine, Trinity College Dublin, 5School of Medicine and CRANN, Trinity College Dublin, 6Nikon AG Instruments

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Resumen
Automatic Translation

Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.

Tags

Harmonic Nanoparticles Regenerative Research In Vitro Labeling Human Embryonic Stem Cells Second Harmonic Generation Nanoparticles Multi-photon Imaging Excitation Light Nonlinear Optical Process HNPs HESC Differentiation Cardiac Clusters Air-liquid Cultures Second Harmonic Emission 3D Monitoring Beating Cardiac Tissue Displacement Patterns
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