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Immunology and Infection

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JoVE Journal Immunology and Infection
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
 

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

Article DOI: 10.3791/52392-v • 07:29 min • December 6th, 2014
December 6th, 2014 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 1,2, 1,4, 1,2
1Molecular Immunology Unit, Université Libre de Bruxelles, 2Institut Jules Bordet, Université Libre de Bruxelles, 3Institut d'Immunologie Médicale, Université Libre de Bruxelles, 4Flow Cytometry Core Facility, Université Libre de Bruxelles
* These authors contributed equally

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This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

Tags

Non-enzymatic Dissociation Fresh Human Tissues Infiltrating Lymphocytes Tumor Escape Immune System Breast Cancer Research Intact Lymphoid Cells Mechanical Dissociator Surface Receptor Expression Enzyme-digested Tissues CD45+ Cells Immunological Characterization Molecular Characterization Subpopulation Purification In Vitro Expansion Cryopreservation Primary Tissue Supernatant
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