Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Development of a Quantitative Recombinase…
JoVE Journal
Biology

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Biology
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
 

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Article DOI: 10.3791/52620-v • 08:37 min • March 30th, 2015
March 30th, 2015 •
*1, *1, 1
1Department of Bioengineering, Rice University
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

Tags

Quantitative Recombinase Polymerase Amplification Assay Internal Positive Control Isothermal Amplification Pathogen Detection DNA Sample Concentration Real-time Quantitative Recombinase Polymerase Amplification Assay HIV-1 DNA Quantification Real-time RPA Reactions Internal Positive Control Sequence Co-amplification Standard Curve Construction Unknown Sample Concentration Prediction Assay Performance Assessment Real-time Fluorescence Data Collection Microscope Stage Heater Point-of-care QRPA Assay
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter