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Immunology and Infection

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JoVE Journal Immunology and Infection
Biomimetic Materials to Characterize Bacteria-host Interactions
 

Biomimetic Materials to Characterize Bacteria-host Interactions

Article DOI: 10.3791/53400-v • 12:22 min • November 16th, 2015
November 16th, 2015 •
*1, *1, 1, 1, 1
1Institute of Microbiology and Infection, School of Biosciences, University of Birmingham
* These authors contributed equally

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Resumen
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Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.

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Biomimetic Materials Bacteria-host Interactions Bacterial Attachment Adhesins Bacterial Colonization Infection Biochemical Characterization Functional Characterization Interference Biomimetic Materials Production Recombinant Adhesins Polystyrene Beads Biochemical Interactions Functional Interactions Host Cell Receptors Coupling Chemistries Polymer Scaffolds Bacteriomimetic Materials Coupling Efficiency Pathogen Mediated Cytotoxicity Scope Limitations Further Applications
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