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Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
 

Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

Article DOI: 10.3791/53741-v • 11:19 min • February 3rd, 2016
February 3rd, 2016 •
*1, *1, 1,2, 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
* These authors contributed equally

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We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.

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Ex Vivo Live Imaging Lung Metastasis Tumor Cell-stroma Interactions Microenvironment Cancer Metastasis Cell Types Visualization Of Live Cells Targeted Therapeutics Lung Section Imaging Cell Death Polystyrene Foam Lid Sterilize The Animal Transverse Epigastric Incision Peritoneum Descending Aorta Vacuum Release Rib Cage Excision
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