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低丰度胚胎标本的染色质沉淀 (芯片) 协议

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Genética

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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

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低丰度胚胎标本的染色质沉淀 (芯片) 协议

DOI:

10.3791/56186-v

•

12:47 min

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August 29, 2017

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Rizwan Rehimi, Michaela Bartusel, Francesca Solinas, Janine Altmüller, Alvaro Rada-Iglesias³

¹Center for Molecular Medicine Cologne (CMMC),University of Cologne, ²Cologne Center for Genomics (CCG),University of Cologne, ³Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne,University of Cologne

Capítulos

00:05Título
00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
04:12Crosslinking Proteins to DNA
05:01Lysis, Sonication, and Immunoprecipitation
06:33Washing, Elution, and Reversal of Crosslinking
08:57Cellular Protein and RNA Digestion
10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
11:48Conclusion

Summary

Traducción Automática

在这里, 我们描述了染色质沉淀 (芯片) 和芯片序列库的准备协议, 以产生全球表剖面从丰鸡胚胎标本。

Tags

Keywords: Chromatin Immunoprecipitation (ChIP)Low-abundance Embryonic Samples Histone Modifications Vertebrate Genomes Biología del desarrollo Enhancers Genes Transcription Regulation Cellular Heterogeneity Patient Samples Chicken Embryos HH19 Extra-embryonic Membranes Spinal Neuro Tube (SNT)Trypsinization DMEM FBS

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