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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
 

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Article DOI: 10.3791/57032-v • 10:22 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University
* These authors contributed equally

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Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.

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In Vitro In Vivo Intestinal Epithelial Cell Permeability Epithelial Barrier Dysfunction Inflammatory Bowel Disease Gastrointestinal Diseases Drug Toxicity Studies Barrier Integrity Caco-2bbe Cells T75 Flask PBS Trypsin-EDTA Incubator
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