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Developmental Biology

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JoVE Journal Developmental Biology
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
 

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Article DOI: 10.3791/57033-v • 10:21 min • February 21st, 2018
February 21st, 2018 •
*1,2, *1, 1, 1,2
1Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University
* These authors contributed equally

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Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.

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Murine Myeloid Precursor 32D/G-CSF-R Cells Genetic Modification Over Expressing Silencing Proliferation Neutrophilic Differentiation Hematology Protein Of Interest Cell Line Unlimited Proliferation Capacity Genetic Manipulations RPMI-1640 Medium Fetal Bovine Serum Interleukin-3 Bosc23 Cells DMEM Medium Retroviral Construct PCL Echo Packaging Vector Polyethylenimine
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