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JoVE Journal Genetics
Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
 

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Article DOI: 10.3791/57889-v • 11:22 min • August 26th, 2018
August 26th, 2018 •
1,2, 1,3, 1, 2, 1,3, 1,3,4
1Department of Genetics and Genome Sciences, Case Western Reserve University, 2Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center, Case Western Reserve University, 4Center for RNA Science and Therapeutics, Case Western Reserve University

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An in situ hybridization (ISH) protocol that uses short antisense oligonucleotides to detect alternative pre-mRNA splicing patterns in mouse brain sections is described.

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Alternative Pre-mRNA Splicing RNA In Situ Hybridization Assay Quantitative Analysis Mouse Brain Sections Shock Anti-static Exon Junction Probes Isoform-specific Hybridization Signals Signal Amplification Technology Exon Inclusion Escaping Specific Probes Splice Variants Anatomical Areas Of Mouse Brain NF1 Exon 23a Expression Patterns Paraffinization Xylene Ethanol
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