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JoVE Journal Developmental Biology
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
 

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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It remains a major challenge to develop conditional gene-knockout or effective gene-knockdown in adult zebrafish organs. Here we report a protocol for performing nanoparticle-mediated siRNA gene-silencing in adult zebrafish heart, thus providing a new loss-of-function method for studying adult organs in zebrafish and other model organisms.

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