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Bioengineering

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JoVE Journal Bioengineering
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
 

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Article DOI: 10.3791/58589-v • 05:30 min • January 23rd, 2019
January 23rd, 2019 •
1, 2, 2, 2, 1,2
1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz

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This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

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Methanol Independent Expression Pichia Pastoris De-repression Technologies Recombinant Proteins Shake Flask Cultivation Up Scaling Methanol-free Protein Expression Online Monitoring Growth Characteristics Glass Grow Feed Yeast Pepto And Dextrose Broth Biomass Measuring Device Bluetooth Connection Computer Monitoring Software Experiment
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