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使用多光谱成像流细胞测定法执行体外微核测定的自动化方法

JoVE Revista
Bioingeniería

This content is Free Access.

JoVE Revista Bioingeniería

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

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使用多光谱成像流细胞测定法执行体外微核测定的自动化方法

DOI:

10.3791/59324-v

•

12:56 min

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May 13, 2019

•

Matthew A. Rodrigues

¹Biology Department,Luminex Corporation

Capítulos

00:04Título
00:33Clastogen and/or Aneugen Exposure
01:49Sample Processing
03:50Multispectral Imaging Flow Cytometry (MIFC)
05:15Binucleated Cell (BNC) Identification
06:48Micronucleus (MN) Identification
07:53Polynucleated Population Identification
08:41BNC and MN Mask Examination
09:52Statistics Table Creation
10:31Experimental File Batch Processing
11:10Results: Representative Cell Scoring and Cytotoxicity Quantification
12:23Conclusion

Summary

Traducción Automática

体外微核测定是评估基因毒性和细胞毒性的成熟方法,但使用人工显微镜对测定进行评分是费力的,具有主观性和分时变异性。本文介绍了为使用多光谱成像流细胞测定执行全自动测试而开发的协议。

Tags

Keywords: In Vitro Micronucleus Assay Multispectral Imaging Flow Cytometry Automated Method Genotoxicity Cytotoxicity Clastogens Aneugens Cell Exposure Cytochalasin B Potassium Chloride Formalin Hoechst 33342 High-throughput Automated Microscopy Conventional Flow Cytometry

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