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Um modelo de câncer endometrial ortotópico com linfadenopatia retroperitoneal feita a partir de in vivo propagadas e cultivadas VX2 células
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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Capítulos
- 00:04Título
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
Este protocolo apresenta um método estandardizado para crescer VX2 pilhas na cultura e para criar um modelo VX2 transplantação orthotopic do do cancro endometrial com metástases de nó de linfa retroperitoneal nos coelhos. Os modelos do cancro endometrial de orthotopic são importantes para o estudo pré-clínico de modalidades novas da imagem latente para o diagnóstico de metástases de nó de linfa.
